New Publication from Dr. Bratman and Dr. de Carvalho
"Circulating cell-free DNA (cfDNA) comprises small DNA fragments derived from normal and tumor tissue that are released into the bloodstream. Recently, methylation proﬁling of cfDNA as a liquid biopsy tool has been gaining prominence due to the presence of tissue-speciﬁc markers in cfDNA. We have previously reported cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) as a sensitive, low-input, cost-efﬁcient and bisulﬁte-free approach to proﬁling DNA methylomes of plasma cfDNA. cfMeDIP-seq is an extension of a previously published MeDIP-seq protocol and is adapted to allow for methylome proﬁling of samples with low input (ranging from 1 to 10 ng) of DNA, which is enabled by the addition of ‘ﬁller DNA’ before immunoprecipitation. This protocol is not limited to plasma cfDNA; it can also be applied to other samples that are naturally sheared and at low availability (e.g., urinary cfDNA and cerebrospinal ﬂuid cfDNA), and is potentially applicable to other applications beyond cancer detection, including prenatal diagnostics, cardiology and monitoring of immune response. The protocol presented here should enable any standard molecular laboratory to generate cfMeDIP-seq libraries from plasma cfDNA in ~3–4d".